HOW IT WORKS
Capture Store Sequence
Work with a wide range of cells, even fragile cells, with no special equipment required
Collect and preserve cells where you find them – in your lab, in the clinic, in the field, at multi-site studies
1 sample = 1 HIVE. Process and analyze your samples individually or all together, with minimal hands-on time
How it works
Collect
Obtain samples (clinical biopsies, solid tissue, organoids, cell culture, etc.) and prepare single-cell suspensions
Capture Cells
Load cells into the HIVE and allow single cells to settle gently into HIVE picowells containing barcoded mRNA-capture beads
The HIVE Difference: Store or Ship
With cells in a stable environment, store HIVEs in the freezer and/or ship when ready to process
Capture Transcripts
Seal the wells to create isolated chambers with one bead and one cell. Lyse cells and allow mRNA to bind to beads
Library Preparation
Extract beads from the HIVE and amplify captured mRNA to create sequencing-ready libraries
Quantify Transcripts
Combine libraries from multiple samples andsequences. Use Honeycomb’s custom BeeNet software to determine the gene expression profiles of single cells
Analyze Data
Standard secondary analysis tools can be used with BeeNet output files to understand the biology of your samples
