The HIVE scRNAseq Benefits

The HIVE expands access to Single-Cell Science to researchers worldwide by eliminating the need for special instruments and allowing cells to be collected and isolated at locations across the hall or across the world from where they are processed. The HIVE minimizes variability and batch effects and enables multi-site studies such as distributed clinical trials, academic collaborations, and service providers with remote customers. The HIVE’s gentle process ensures capture of all the cells in the sample, and can accommodate a range of loading volumes from 50 microliters to 4 mL

See All Your Cells

Recover complete biological information from your sample, even from fragile or delicate cells

Cells isolated using the HIVE settle gently into microwells, avoiding elevated pressures or microfluidic shear forces common in other methods.

Example

A researcher has been attempting to recover neutrophils from whole blood clinical samples. He has been struggling to recover neutrophils with current microfluidic platforms in the lab, but the HIVE’s gentle capture of cells enables him to generate data for neutrophils and other fragile cell types.

Any where, Any time

Capture cells wherever and whenever they are available without compromising data quality

The upper plots show the complete recovery of all cell types from HIVEs processed on Day 0 (fresh) or Day 14 (stored at -20C). The bar plot shows that the relative frequency of each cell type is consistent between these timepoints.

Example

A clinical researcher receives bone marrow biopsies at odd hours, making it challenging to process them fresh and at the same time for ideal data quality. Additionally, the specialized equipment required for her current single cell solution is not always available. With the HIVE, she can capture her samples at any time, then store her cell-loaded HIVE(s) until all samples are ready for processing together, as if they had been processed fresh.

Flexible Sample Handling

Run one or many samples in parallel, in a wide range of volumes

The HIVE workflow accommodates a broad range of sample numbers and volumes.

Minimal impact in overall time and user experience running anywhere from 1 to 24 samples in parallel. From single cells to NGS libraries only takes 1.5 hours longer for 24 HIVEs vs. 1 HIVE.

Limited constraints on sample loading volume. Load between 1 and 4 mLs per HIVE. Top up samples < 1 mL with sample buffer or sequentially load samples > 4 mL.

Example

A researcher has 10 mL of fresh urine with only 10,000 cells total. With their current single-cell technology, they have to concentrate 10 mLs down to tens of uLs. With the HIVE, they can load their entire sample without any risk of losing cells due to concentration before capture.